Phenotype and in vitro function of mature MDDC generated from cryopreserved PBMC of cancer patients are equivalent to those from healthy donors |
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| Authors: | Smita A Ghanekar Sonny Bhatia Joyce J Ruitenberg DeLa Corazon Rosa Mary L Disis Vernon C Maino Holden T Maecker Cory A Waters |
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| Affiliation: | (1) BD Biosciences Immunocytometry Systems, 2350 Qume Dr., San Jose, CA 95131, USA;(2) Division of Oncology, University of Washington, 815 Mercer St., Seattle, WA 98109, USA |
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| Abstract: | Background Monocyte-derived-dendritic-cells (MDDC) are the major DC type used in vaccine-based clinical studies for a variety of cancers. In order to assess whether in vitro differentiated MDDC from cryopreserved PBMC of cancer patients are functionally distinct from those of healthy donors, we compared these cells for their expression of co-stimulatory and functional markers. In addition, the effect of cryopreservation of PBMC precursors on the quality of MDDC was also evaluated using samples from healthy donors. |
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