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Selective cell culture on UV transparent polymer by F2 laser surface modification |
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| Authors: | Yasutaka Hanada Koji Sugioka Takayoshi Tsuchimoto Iwao Miyamoto Katsumi Midorikawa |
| Affiliation: | a RIKEN-Advanced Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan b RIKEN-Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan c Department of Applied Electronics, Faculty of Industrial Science & Technology Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan |
| Abstract: | A microchip made of UV transparent polymer (CYTOP) that can perform selective cell culture has been fabricated by F2 laser surface modification. The refractive index of CYTOP is almost the same as that of culture medium, which is essential for three-dimensional (3D) observation of cells. The F2 laser modification of CYTOP achieves hydrophilicity only on the laser irradiated area with little deterioration of the optical properties and surface smoothness. After the laser modification, HeLa cells were successfully cultured and strongly adhered only on the modified area of CYTOP. The cells patterned on CYTOP were applied for clear 3D observation using an optical microscope in phase contrast mode. |
| Keywords: | 87.85.Lf 81.65.&minus b 61.80.Ba |
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