Fluorescence enhancement assay for trace iron(II) using Pyr-tempo as a spin label fluorescent probe |
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Authors: | Jin-Long Chen Chang-Qing Zhu |
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Institution: | (1) College of Chemistry and Materials Science, Anhui Key Laboratory of Functional Molecular Solids, Anhui Normal University, 241000 Wuhu, P.R. China;(2) School of Chemistry and Environmental Science, AnQing Normal College, 246003 AnQing, P.R. China |
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Abstract: | Pyrene-tetramethylpiperidinyl (Pyr-Tempo) as a spin label fluorescent probe for iron(II) was synthesized. It exhibited weak
fluorescence (λexc/λem = 346/399 nm) in aqueous solution due to an intramolecular quenching pathway. A method for determination of iron(II) was
proposed based on the fluorescence enhancement of the probe in the presence of iron(II) in acidic medium. Under optimum conditions,
the fluorescence enhancement of Pyr-Tempo is linearly proportional to the iron(II) concentration range of 6.0 × 10−8 to 9.6 × 10−6 mol L−1 with a detection limit of 8.0 × 10−9 mol L−1. The relative standard deviation (RSD) of six replicate measurements is 1.95% for 3.0 × 10−7 mol L−1 iron(II). The developed spin label fluorescence probe is found to be rapidly and sensitively responsive to iron(II) with
high selectivity compared to existing fluorescence methods. The proposed method was successfully applied to iron(II) detection
in five real samples with satisfactory results obtained by manual UV/Vis spectrophotometry (standard method) with 1,10-phenanthroline. |
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Keywords: | : Fluorescence spin-label iron(II) |
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