| Study on the Interaction between Distamycin Analogs and DNA from Herring Sperm by Fluorimetry |
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| 作者姓名： | Han LIANG Jing Jian LI* Xiao Dong LI Fei Li TANG Gu YUAN* |
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| 作者单位： | Han LIANG1，Jing Jian LI1*，Xiao Dong LI2，Fei Li TANG2，Gu YUAN2* 1Department of Chemistry，Peking University，Beijing 100871 2The Key Laboratory of Bioorganic Chemistry and Molecular Engineering，Department of Chemical Biology，Peking University，Beij |
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| 摘 要： | Distamycin is a naturally occurring antibiotic which binds to the AT rich regions in minor groove of DNA1. We have reported the interaction between the analogs and calf thymus DNA by circular dichroism spectropolarimetry (CD) and isothermal titration calorimetry (ITC)2. Due to the greater sensitivity of fluorescence-based techniques in comparison with CD, we employ the fluorimety to explore the interaction between four new distamycin analogs and DNA from herring sperm. O2NNNOHNNO…
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Study on the interaction between distamycin analogs and DNA from herr-ing sperm by fluorimetry |
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| Han LIANG,Jing Jian LI*,Xiao Dong LI,Fei Li TANG,Gu YUAN* .Study on the Interaction between Distamycin Analogs and DNA from Herring Sperm by Fluorimetry[J].Chinese Chemical Letters,2002,13(10). |
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| Authors: | Han LIANG Jing Jian LI Xiao Dong LI Fei Li TANG GU Yuan |
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| Institution: | Department of Chemistry, Peking University, Beijing 100871 Department of Chemistry, Peking University, Beijing 100871 The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Department of Chemical Biology, Peking University, Berjing 100871 The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Department of Chemical Biology, Peking University, Berjing 100871 The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Department of Chemical Biology, Peking University, Berjing 100871 |
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| Abstract: | The fluorescence spectra of the interaction between four new distamycin analogs and DNA from herring sperm have been studied in detail. The fluorescence quenching of the DNA by the analogs was observed, and the fluorescence intensity of the DNA was attenuated exponentially with the increasing of the analogs concentration. |
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| Keywords: | Distamycin analogs fluorescence quenching DNA from herring sperm |
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