Capillary electrophoretic studies on quantum dots and histidine appended peptides self‐assembly |
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Authors: | Jianhao Wang Jingyan Li Yao Chen Yiwan Teng Cheli Wang Jinchen Li Li Liu Bingyu Dong Lin Qiu Pengju Jiang |
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Institution: | School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China |
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Abstract: | Herein, we designed four peptides appended with different numbers of histidine (Hisn‐peptide). We launched a systematic investigation on quantum dots (QDs) and Hisn‐peptide self‐assembly in solution using fluorescence coupled CE (CE‐FL). The results indicated that CE‐FL was a powerful method to probe how ligands interaction on the surface of nanoparticles. The self‐assembly of QDs and peptide was determined by the numbers of histidine. We also observed that longer polyhistidine tags (n ≤ 6) could improve the self‐assembly efficiency. Furthermore, the formation and separation of QD‐peptide assembly were also studied by CE‐FL inside a capillary. The total time for the mixing, self‐assembly, separation, and detection was less than 10 min. Our method greatly expands the application of CE‐FL in QDs‐based biolabeling and bioanalysis. |
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Keywords: | CE‐FL Hisn‐peptide In‐capillary Quantum dots Self‐assembly |
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