Study of the enantioseparation capability of chiral dual system based on chondroitin sulfate C in CE |
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Authors: | Jiaquan Chen Yingxiang Du Fenxia Zhu Bin Chen Qi Zhang Shuaijing Du Ping Li |
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Institution: | 1. Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, P. R. China;2. Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing, P. R. China;3. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, P. R. China;4. Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, P. R. China;5. Department of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, P. R. China;6. College of Chemistry and Molecular Engineering, Peking University, Beijing, P. R. China |
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Abstract: | It has been reported that chiral dual system is able to improve the enantioseparation of enantiomers in many cases. Currently, the dual systems involved in CE chiral separation are mostly dual CDs systems, and the polysaccharides‐based chiral dual system was reported in only one paper. To the best of our knowledge, the use of chondroitin sulfate C (CSC)‐based dual system for enantiomeric separation has not been reported previously. Herein, four CSC‐based chiral dual systems, namely CSC/glycogen, CSC/chondroitin sulfate A (CSA), CSC/hydroxypropyl‐β‐CD (HP‐β‐CD), as well as CSC/β‐CD (β‐CD), were evaluated for the first time for their enantioseparation capability by CE in this paper. During the course of the work, the influences of chiral selector concentration and buffer pH values on enantioseparation in dual systems were systematically investigated. Under the optimized conditions, the dual system consisting of CSC and glycogen exhibited better separations toward nefopam, duloxetine, sulconazole, atenolol, laudanosine, and cetirizine enantiomers compared to the single CSC or glycogen system. The combination of CSC and HP‐β‐CD improved the separation of amlodipine and chlorphenamine enantiomers. However, no synergistic effect was observed in the CSC/CSA and CSC/β‐CD systems. |
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Keywords: | Capillary electrophoresis Chiral dual system Chondroitin sulfate C Enantioseparation Glycogen |
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