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Voltammetric Behaviour of 7‐Methylguanine Using Screen‐printed Graphite Electrodes: towards a Guanine Methylation Electrochemical Sensor |
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| Authors: | Ariadna Brotons Ignacio Sanjuan Craig E. Banks Francisco José Vidal‐Iglesias José Solla‐Gullón Jesús Iniesta |
| Affiliation: | 1. Institute of Electrochemistry, University of Alicante, 03690, San Vicente del Raspeig, Alicante, Spain, Tel: +34?965909850;2. Faculty of Science and Engineering, School of Chemistry and the Environment, Division of Chemistry and Environmental Science, Manchester Metropolitan University, Chester Street, Manchester M1?5GD, Lancs, UK;3. Physical Chemistry Department, University of Alicante, 03690, San Vicente del Raspeig, Alicante, Spain |
| Abstract: | The determination of 7‐methylguanine (7mG) is an interesting analytical target since its quantification can be correlated with the methylation of cytosine which is one of the most relevant epigenetic modification of DNA. In this work, screen‐printed graphite electrodes were used to study the electrochemical characterization of 7mG by cyclic and square wave voltammetry. 7mG concentration and pH were examined, as well as the repeatability of the measurements. The electrochemical response of 7mG in the presence of guanine and adenine was also investigated. Results aim to the future development of an electrochemical sensor for the methylation degree in DNA. |
| Keywords: | Screen printed graphite electrode guanine 7‐methylguanine adenine DNA methylation |