A Solid‐State Electrochemiluminescence Immunosensor Based on MWCNTs‐Nafion and Ru(bpy)32+/Nano‐Pt Nanocomposites for Detection of α‐Fetoprotein |
| |
Authors: | Yaling Cao Ruo Yuan Yaqin Chai Li Mao Xia Yang Shirong Yuan Yali Yuan Yuhong Liao |
| |
Institution: | Key Laboratory on Luminescence and Real‐Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P.?R. China |
| |
Abstract: | A approach was successfully employed for constructing a solid‐state electrochemiluminescence (ECL) immunosensor by layer‐by‐layer self‐assembly of multiwall carbon nanotubes (MWCNTs)‐Nafion composite film, Ru(bpy)32+/nano‐Pt aggregates (Ru‐PtNPs) and Pt nanoparticles (PtNPs). The influence of Pt nanoparticles on the ECL intensity was quantitatively evaluated by calculating the electroactive surface area of different electrodes with or without PtNPs to immobilize Ru(bpy)32+. The principle of ECL detection for target α‐fetoprotein antigen (AFP) was based on the increment of resistance after immunoreaction, which led to a decrease in ECL intensity. The linear response range was 0.01–10 ng mL?1 with the detection limit of 3.3 pg mL?1. The immunosensor exhibited advantages of simple preparation and operation, high sensitivity and good selectivity. |
| |
Keywords: | Electrochemiluminescence (ECL) Ru(bpy)32+ Ru‐PtNPs Electroactive surface area α ‐Fetoprotein antigen (AFP) |
|