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The Thermophilic CotA Laccase from Bacillus subtilis: Bioelectrocatalytic Evaluation of O2 Reduction in the Direct and Mediated Electron Transfer Regime |
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| Authors: | Dmitrii A Guschin Andrew D Griffiths Valerie Taly Wolfgang Schuhmann |
| Institution: | 1. Analytische Chemie – Elektroanalytik & Sensorik, Ruhr‐Universit?t Bochum, Universit?tsstr. 150, D‐44780 Bochum, Germany;2. Center for Electrochemical Sciences – CES, Ruhr‐Universit?t Bochum, Universit?tsstr. 150, D‐44780 Bochum, Germany;3. Institut de Science et d'Ingénierie Supramoléculaires (ISIS), CNRS UMR 7006, 8 allée Gaspard Monge, 67083 Strasbourg, France |
| Abstract: | The thermophilic bacterial laccase CotA from Bacillus subtilis adsorbed on graphite electrodes enables monitoring of the temperature dependent direct biocatalytic O2 reduction. Its entrapment in two different Os‐complex modified redox hydrogels is the basis for mediated bioelectroreduction of O2. Besides the temperature and pH dependence of the bioelectrocatalytic response chloride and fluoride inhibition studies demonstrate that the Os‐complexes are bound to the T1 copper centre of the enzyme. The interaction between CotA and the Os‐complex modified polymers prevents inhibition by chloride ions. |
| Keywords: | CotA laccase Direct electron transfer Halide inhibition Mediated electron transfer Osmium‐complex modified polymers |