Voltammetry and Spectroscopy Study of In Vitro Interaction of Fenitrothion with DNA

The interaction of Fenitrothion with DNA has been studied using the voltammetric and spectroscopic methods. An irreversible reduction was concluded from the CV data of Fenitrothion. The values of (αn)_f=1.91 and (αn)_b=2.06 were measured and the reduction process of Fenitrothion was performed with 4 electrons. By using non‐linear regression analysis of CV data, the binding constant, binding site size, and diffusion coefficient for free Fenitrothion (D_f) and DNA‐Fenitrothion (D_b) were calculated as: 1.03×10⁴, 1.204, 5.2×10^?4 and 1.72×10^?5, respectively. Also the voltammetric, spectroscopic, thermodynamic and activation energy of DNA‐Fenitrothion complex revealed that the mode of interaction might be partial‐intercalative and complex formation is entropy favored.