Simultaneous Quantification of Multiple Cancer Biomarkers in Blood Samples through DNA‐Assisted Nanopore Sensing |
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| Authors: | Dr Lei Liu Ting Li Dr Shouwen Zhang Dr Peng Song Bingyuan Guo Prof Yuliang Zhao Prof Hai‐Chen Wu |
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| Institution: | 1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China;2. Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, China;3. Epilepsy Department, Beijing ChaoYang Emergency Medical Center, Beijing, China;4. Department of Geriatric Oncology, General Hospital of the Chinese People's Liberation Army, Beijing, China |
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| Abstract: | Protein biomarkers in blood have been widely used in the early diagnosis of disease. However, simultaneous detection of many biomarkers in a single sample remains challenging. Herein, we show that the combination of a sandwich assay and DNA‐assisted nanopore sensing could unambiguously identify and quantify several antigens in a mixture. We use five barcode DNAs to label different gold nanoparticles that can selectively bind specific antigens. After the completion of the sandwich assay, barcode DNAs are released and subject to nanopore translocation tests. The distinct current signatures generated by each barcode DNA allow simultaneous quantification of biomarkers at picomolar level in clinical samples. This approach would be very useful for accurate and multiplexed quantification of cancer‐associated biomarkers within a very small sample volume, which is critical for non‐invasive early diagnosis of cancer. |
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| Keywords: | antigens barcode DNA biomarkers multiplexed detection nanopore sensing |
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