Direct electrochemistry and electrocatalysis of horseradish peroxidase immobilized in Nafion-RTIL composite film |
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| Institution: | 1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China;2. Graduate School of the Chinese Academy of Sciences, Bejing, PR China;1. School of Chemistry and Environment, South China Normal University, Guangzhou 510006, China;2. Key Laboratory of Electrochemical Technology on Energy Storage and Power Generation of Guangdong Higher Education Institutes, South China Normal University, Guangzhou 510006, China;3. Engineering Research Center of Materials and Technology for Electrochemical Energy Storage (Ministry of Education), South China Normal University, Guangzhou 510006, China;1. Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu Province, Wuxi 214122, China;2. Fiber and Polymer Science Program, North Carolina State University, 2401 Research Drive, Campus Box 8301, Raleigh, NC 27695-8301, United States;3. Department of Chemistry, North Carolina State University, 2620 Yarbrough Drive, Raleigh, NC 27695-8204, United States;4. Department of Forest Biomaterials, North Carolina State University, 2820 Faucette Drive, Campus Box 8005, Raleigh, NC 27695-8005, United States;5. Qilu University of Technology, Key Laboratory of Pulp & Paper Science and Technology of the Ministry of Education, Jinan 250353, PR China;1. College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, China;2. School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu 730070, China;3. Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China;4. Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, Australia;1. Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, 09340 México D.F., Mexico;2. Grupo de Procesos de Transporte y Reacción en Sistemas Multifásicos, Departamento de IPH, Universidad Autónoma Metropolitana-Iztapalapa, México D.F., Mexico;3. Departamento de Ingeniería Química, Universidad del Valle de México-Coyoacan, Calz de Tlalpan 122, Col. Tlalpan, 09979 México D.F., Mexico;4. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Normalistas 800, Guadalajara, Jalisco, Mexico;5. Department of Biochemical Engineering, University College London, London WC1E 7JE, United Kingdom;1. Institute of Applied Synthetic Chemistry, TU Wien, Getreidemarkt 9/163-OC, A-1060 Vienna, Austria;2. Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok körútja 2, 117 Budapest, Hungary;3. Institute of Chemical Technologies and Analytics, TU Wien, Getreidemarkt 9/164-SC, A-1060 Vienna, Austria |
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| Abstract: | The composite film based on Nafion and hydrophobic room-temperature ionic liquid (RTIL) 1-butyl-3-methyl-imidazolium hexafluorophosphate (bmim] PF6) was explored. Here, Nafion was used as a binder to form Nafion-ionic liquids composite film and help bmim] PF6 effectively adhered on glassy carbon (GC) electrode. X-ray photoelectron spectroscopy (XPS), cyclic voltammtery (CV) and electrochemical impedance spectroscopy (EIS) were used to characterize this composite film, showing that the composite film can effectively adhere on the GC electrode surface through Nafion interacting with bmim] PF6 and GC electrode. Meanwhile, doping bmim] PF6 in Nafion can also effectively reduce the electron transfer resistance of Nafion. The composite film can be readily used as an immobilization matrix to entrap horseradish peroxidase (HRP). A pair of well-defined redox peaks of HRP was obtained at the HRP/Nafion-bmim] PF6 composite film-modified GC electrode through direct electron transfer between the protein and the underlying electrode. HRP can still retain its biological activity and enhance electrochemical reduction towards O2 and H2O2. It is expected that this composite film may find more potential applications in biosensors and biocatalysis. |
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