Specific identification of Bacillus anthracis strains |
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| Institution: | 1. Edgewood Chemical and Biological Center, Aberdeen Proving Ground, MD 21010-5424, USA;2. Science & Technology Corporation, Edgewood, MD 21040, USA;3. Scripps Research Institute, La Jolla, CA 92037, USA;1. Chemistry Division, Bhabha Atomic Research Centre, Mumbai 400085, India;2. Department of Chemistry, University of Pune, Pune 411007, India;1. Laboratory Medical Optical and Mass Spectrometry, Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, No. 350, Shushanhu Rd., Hefei 230031, China;2. Laboratory of Environmental Spectroscopy, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, No. 350, Shushanhu Rd., Hefei 230031, China;3. School of Science, Anhui Agricultural University, Hefei 230036, China;1. Unité de Recherche Chimie de l’Environnement et Moléculaire, Structurale ‘CHEMS’, Faculté des Sciences Exactes, Campus Chaabet Ersas, Université Frères Mentouri Constantine 1, 25000 Constantine, Algeria;2. LCC-CNRS, Université de Toulouse, CNRS, UPS, INPT, Toulouse, France;3. IUT A Paul Sabatier, Dpt. de Chimie, Ave. G. Pompidou, F-81104 Castres Cedex, France;1. Boreskov Institute of Catalysis, Pr. Lavrentieva, 5, Novosibirsk 630090, Russia;2. Novosibirsk State University, Pirogova St., 2, Novosibirsk 630090, Russia;1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China;2. Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, Columbus, OH, United States |
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| Abstract: | Accurate identification of human pathogens is the initial vital step in treating the civilian terrorism victims and military personnel afflicted in biological threat situations. We have applied a powerful multi-dimensional protein identification technology (MudPIT) along with newly generated software termed Profiler to identify the sequences of specific proteins observed for few strains of Bacillus anthracis, a human pathogen. Software termed Profiler was created to initially screen the MudPIT data of B. anthracis strains and establish the observed proteins specific for its strains. A database was also generated using Profiler containing marker proteins of B. anthracis and its strains, which in turn could be used for detecting the organism and its corresponding strains in samples. Analysis of the unknowns by our methodology, combining MudPIT and Profiler, led to the accurate identification of the anthracis strains present in samples. Thus, a new approach for the identification of B. anthracis strains in unknown samples, based on the molecular mass and sequences of marker proteins, has been ascertained. |
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