探针5-氨基水杨酸锌测定蛋白质的应用研究

在模拟人体生理条件下，基于5-氨基水杨酸锌(5-ASZ)与血清白蛋白相互作用生成复合物，导致血清白蛋白的内源荧光产生特异性变化，从而建立了以5-ASZ为荧光探针，用固定波长同步荧光光谱分析测定蛋白质(HSA)的新方法。同步荧光光谱特征及强度与Δλ值、反应介质、反应温度等因素有关。在最佳实验条件下，体系的同步荧光强度(I_SF)与人血清白蛋白在1.66～496.8 μg·mL-1 范围内呈良好的线性关系,检测限为0.88 μg·mL-1(n=11)。在此基础上对血清、尿样和唾液中的蛋白质进行了测定，加标回收率在98.0 %～103.8%之间。实验结果表明：该方法具有简单、快速、灵敏度较高、线性范围宽、精密度和回收率较好等优点。该法直接用于样品中蛋白质总量的测定，获得了令人满意的结果，有望用于生化分析和临床分析领域。

Studied on the Determination of Protein by Probe of Zinc 5-Aminosalicylate

Zinc 5- aminosalylicylate (5-ASZ), a new creative medicine, can react with serum albumin to form a complex. Based on this, a new method for the determination of proteins by synchronous fluorescence spectra under simulated physiological conditions was established using 5 - ASZ as a fluorescence probe. The spectral characterization and intensity of synchronous fluorescence were related to the value of deltalambda, reaction medium and reaction temperature, and so on. On basis of these, eperimental results show that, under the optimum conditions, the synchronous fluorescence intensity of the system is in proportion to the concentration of protein in the range of 1.66-496.8 microg x mL(-1) for human serum albumin (HSA). The detection limit was 0.88 microg x mL(-1) (n = 11) for HSA. On basis of these it had been applied to the determination of proteins in human serum, urine and spit samples with satisfactory results, the recovery was within the range of 98.0%-103.8%. Results suggested that the method possessed easy of implementation, rapidity, high sensitivity, broad linear range, and better RSD and recovery, and so on, and the method was employed directly to determine the total proteins in samples, which results were satisfactory.