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Mesoporous silica hollow sphere (MSHS) for the bioelectrochemistry of horseradish peroxidase |
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| Authors: | Zhaoxia Cao Jian Zhang Julan Zeng Lixian Sun Fen Xu Zhong Cao Daowu Yang |
| Affiliation: | a Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Materials & Thermochemistry Laboratory, 457 Zhongshan Road, Dalian 116023, Liaoning Province, China b Graduate School of the Chinese Academy of Sciences, Beijing 100049, China c School of Chemistry and Environmental Engineering, Changsha University of Science and Technology, Changsha 410076, China |
| Abstract: | In this work, novel mesoporous silica hollow spheres (MSHS) were chosen as an immobilization matrix, to construct a mediator-free third-generation HRP biosensor. UV-vis spectroscopy revealed that horseradish peroxidase (HRP) entrapped in MSHS could retain its native structure. FTIR spectroscopy and nitrogen adsorption-desorption isotherms indicated that HRP are intercalated into the mesopores. The direct electron transfer of HRP entrapped in MSHS was observed. A pair of stable and well-defined redox peaks of HRP with a formal potential of about −0.150 V (vs. Ag/AgCl) in 0.1 M pH 7.0 phosphate-buffered solution (PBS) were obtained. The biosensor exhibited a fast amperometric response to H2O2 with a linear range of 3.9 × 10−6 to 1.4 × 10−4 M (R = 0.997, N = 20). The detection limit was 1.2 × 10−6 M based S/N = 3. |
| Keywords: | Hollow mesoporous silica spheres Horseradish peroxidase Biosensor Direct electron transfer |
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