An electrochemiluminescent biosensor for glucose based on the electrochemiluminescence of luminol on the nafion/glucose oxidase/poly(nickel(II)tetrasulfophthalocyanine)/multi-walled carbon nanotubes modified electrode |
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| Authors: | Bin Qiu Zhenyu Lin Jian Wang Zhihuang Chen Jinhua Chen Guonan Chen |
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| Institution: | a Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), Fuzhou, Fujian 350002, China
b Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China |
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| Abstract: | A poly(nickel(II) tetrasulfophthalocyanine)/multi-walled carbon nanotubes composite modified electrode (polyNiTSPc/MWNTs) was fabricated by electropolymerization of NiTSPc on MWNTs-modified glassy carbon electrode (GCE). The modified electrode was found to be able to greatly improve the emission of luminol electrochemiluminescence (ECL) in a solution containing hydrogen peroxide. Glucose oxidase (GOD) was immobilized on the surface of polyNiTSPc/MWNTs modified GC electrode by Nafion to establish an ECL glucose sensor. Under the optimum conditions, the linear response range of glucose was 1.0 × 10−6 to 1.0 × 10−4 mol L−1 with a detection limit of 8.0 × 10−8 mol L−1 (defined as the concentration that could be detected at the signal-to-noise ratio of 3). The ECL sensor showed an outstanding well reproducibility and long-term stability. The established method has been applied to determine the glucose concentrations in real serum samples with satisfactory results. |
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| Keywords: | Electrochemiluminescent sensor Glucose Nickel phthalocyanine Multi-walled carbon nanotubes Luminol |
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