| Determination of Melatonin in Rat Pineal Gland and Drug with Flow-Injection Chemiluminescence |
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| 作者姓名： | 韦寿莲 赵利霞 程祥磊 林金明 |
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| 作者单位： | [1]Department of Light Industry ＆ Chemistry, Zhaoqing University, Zhaoqing, Guangdong 526061, China [2]Department of Chemistry, Tsinghua University, Beijing 100084, China [3]State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China （No. 20437020） and Zhaoqing Innovative Research Program. |
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| 摘 要： | A flow-injection chemiluminescence （CL） method for the determination of melatonin based on the CL reaction of melatonin with hydrogen peroxide and sodium hypochlorite （NaOCl） in a basic alkaline solution was developed. The possible CL mechanism has been discussed, and a proposal for the reaction pathway was given that singlet oxygen was clarified to be produced in this reaction system and was responsible for the CL emission. Under the optimized conditions, the linear concentration range of application was 1.0×10^-7-2.5 × 10^-4 moloL-I with a de- tection limit of 5.0 ×10^-8 moloL-1 （S/N= 3）. The relative standard deviation for eight repeated measurements of 1.0×10^-6 mol·L^-1 melatonin was 2.8%. The interferences of several important biological substances, some indole compound, cations and anions were studied. No interference was found for the anions, glucose, starch, most of cations and low concentration （less than 3.0 × 10^-6 mol·L^-1） of some biological substances and indole compound. The method was applied to the determination of melatonin in rat pineal gland and drug with satisfactory results. The sample throughput was 90 injections per hour.
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| 关 键 词： | 大鼠 松果腺 药物 褪黑激素 流动注射化学发光分析 |
| 修稿时间： | 2006-03-102006-12-15 |
Determination of Melatonin in Rat Pineal Gland and Drug with Flow-Injection Chemiluminescence |
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| WEI Shou-Lian, ZHAO Li-Xia, CHENG Xiang-Lei, LIN Jin-Ming.Determination of Melatonin in Rat Pineal Gland and Drug with Flow-Injection Chemiluminescence[J].Chinese Journal of Chemistry,2007,25(4):535-541. |
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| Authors: | WEI Shou-Lian ZHAO Li-Xia CHENG Xiang-Lei LIN Jin-Ming |
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| Institution: | [1]Department of Light Industry & Chemistry, Zhaoqing University, Zhaoqing, Guangdong 526061, China; [2]Department of Chemistry, Tsinghua University, Beijing 100084, China; [3]State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China |
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| Abstract: | A flow‐injection chemiluminescence (CL) method for the determination of melatonin based on the CL reaction of melatonin with hydrogen peroxide and sodium hypochlorite (NaOCl) in a basic alkaline solution was developed. The possible CL mechanism has been discussed, and a proposal for the reaction pathway was given that singlet oxygen was clarified to be produced in this reaction system and was responsible for the CL emission. Under the optimized conditions, the linear concentration range of application was 1.0×10?7–2.5×10?4 mol·L?1 with a detection limit of 5.0×10?8 mol·L?1 (S/N=3). The relative standard deviation for eight repeated measurements of 1.0×10?6 mol·L?1 melatonin was 2.8%. The interferences of several important biological substances, some indole compound, cations and anions were studied. No interference was found for the anions, glucose, starch, most of cations and low concentration (less than 3.0×10?6 mol·L?1) of some biological substances and indole compound. The method was applied to the determination of melatonin in rat pineal gland and drug with satisfactory results. The sample throughput was 90 injections per hour. |
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| Keywords: | chemiluminescence melatonin hypochlorite rat pineal gland |
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