| Study on the Interaction of Colloidal Gold with Taq DNA Polymerase |
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| 引用本文： | 朱红平 米丽娟 陈仕谋 王文锋 姚思德. Study on the Interaction of Colloidal Gold with Taq DNA Polymerase[J]. 中国化学, 2007, 25(9): 1233-1237. DOI: 10.1002/cjoc.200790229 |
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| 作者姓名： | 朱红平 米丽娟 陈仕谋 王文锋 姚思德 |
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| 作者单位： | [1]Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Shanghai201800,China [2]Graduate University of Chinese Academ of Sciences,Beijing100049,China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China （No. 10675158）. |
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| 摘 要： | The interaction of colloidal gold with Taq DNA polymerase （Taq） was investigated in this study. Taq-gold conjugate was formed by adding the enzyme to the colloidal gold solution, as evidenced by UV-Vis spectroscopy, X-ray photoelectron spectroscopy, and photon cross correlation spectroscopy measurements. The conjugate was further characterized by transmission electron microscopy. It was found that the Taq-gold conjugate particles were still spherical and well-dispersed. The influence of gold nanoparticles on the bioactivity of Taq was studied by analyzing the yield of the polymerase chain reaction amplification. Results indicated that the enzymatic activity of Taq decreased after interaction with the colloidal gold.
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| 关 键 词： | 胶体金 DNA 聚合酶 共轭化合物 |
| 修稿时间： | 2006-11-21 |
Study on the Interaction of Colloidal Gold with Taq DNA Polymerase |
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| ZHU Hong-Pingt,MI Li-Juan,CHEN Shi-Mou,WANG Wen-Feng,YAO Si-De,. Study on the Interaction of Colloidal Gold with Taq DNA Polymerase[J]. Chinese Journal of Chemistry, 2007, 25(9): 1233-1237. DOI: 10.1002/cjoc.200790229 |
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| Authors: | ZHU Hong-Pingt MI Li-Juan CHEN Shi-Mou WANG Wen-Feng YAO Si-De |
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| Affiliation: | 1.Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China ;2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | The interaction of colloidal gold with Taq DNA polymerase (Taq) was investigated in this study. Taq‐gold conjugate was formed by adding the enzyme to the colloidal gold solution, as evidenced by UV‐Vis spectroscopy, X‐ray photoelectron spectroscopy, and photon cross correlation spectroscopy measurements. The conjugate was further characterized by transmission electron microscopy. It was found that the Taq‐gold conjugate particles were still spherical and well‐dispersed. The influence of gold nanoparticles on the bioactivity of Taq was studied by analyzing the yield of the polymerase chain reaction amplification. Results indicated that the enzymatic activity of Taq decreased after interaction with the colloidal gold. |
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| Keywords: | Taq DNA polymerase colloidal gold conjugate biocatalytic activity polymerase chain reaction |
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