| Amperometric Sensor Based on Neutral Red-Doped Silica Nanoparticles Coupled with Microdialysis for the Measurement of Glutamate in the Rat Striatum |
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| 作者姓名： | 李晓华 鲜跃仲 谢宗红 闵红 李陈鑫 金利通 |
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| 作者单位： | Department of Chemistry, East China Normal University, Shanghai 200062, China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China （Nos. 20175006, 20305007） and Doctoral Foundation of China Ministry of Education （No. 20030269014）. |
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| 摘 要： | Amperometric sensor based on neutral red-doped silica （NRSiO2） nanoparticles （NPs） was fabricated and coupled with a microdialysis sampling system
for the detection of glutamate （Glu） in the rat striatum. The NRSiO2 NPs about （45 ± 3） nm] were prepared with water-in-oil （W/O） microemulsion method, and characterized by transmission electron microscope （TEM） technique. The neutral red （NR） doped in silica network could maintain its high electroactivity and behave as an excellent electron mediator for electrocatalysis of hydrogen dioxide. Furthermore, the silica surface could prevent the leakage of NR, hence, the stability of biosensor was improved. The novel Glu biosensor showed a linear range from 5.0×10^-7 to 1.5×10^-4 mol/L, with a detection limit of 2.0×10^-7 mol/L （S/N=3）.
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| 关 键 词： | 谷氨酸盐氧化酶 生物传感器 鼠纹状体 电流计传感器 |
| 修稿时间： | 2006-05-232007-03-22 |
Amperometric Sensor Based on Neutral Red-Doped Silica Nanoparticles Coupled with Microdialysis for the Measurement of Glutamate in the Rat Striatum |
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| LI Xiao-Hua, XIAN Yue-Zhong,XIE Zong-Hong, MIN Hong, LI Chen-Xin, JIN Li-Tong.Amperometric Sensor Based on Neutral Red-Doped Silica Nanoparticles Coupled with Microdialysis for the Measurement of Glutamate in the Rat Striatum[J].Chinese Journal of Chemistry,2007,25(7):953-957. |
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| Authors: | LI Xiao-Hua XIAN Yue-Zhong XIE Zong-Hong MIN Hong LI Chen-Xin JIN Li-Tong |
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| Institution: | Department of Chemistry, East China Normal University, Shanghai 200062, China |
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| Abstract: | Amperometric sensor based on neutral red‐doped silica (NRSiO2) nanoparticles (NPs) was fabricated and coupled with a microdialysis sampling system for the detection of glutamate (Glu) in the rat striatum. The NRSiO2 NPs about (45±3) nm] were prepared with water‐in‐oil (W/O) microemulsion method, and characterized by transmission electron microscope (TEM) technique. The neutral red (NR) doped in silica network could maintain its high electroactivity and behave as an excellent electron mediator for electrocatalysis of hydrogen dioxide. Furthermore, the silica surface could prevent the leakage of NR, hence, the stability of biosensor was improved. The novel Glu biosensor showed a linear range from 5.0×10?7 to 1.5×10?4 mol/L, with a detection limit of 2.0×10?7 mol/L(S/N?3). |
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| Keywords: | NRSiO2 glutamate oxidase （GluOx） glutamate biosensor microdialysis rat striatum |
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