| A Highly Sensitive Spectrophotometric Assay of Bleomycins Based on the Fading Reaction of Some Halofluorescein Dyes |
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| 作者姓名： | 刘江涛 刘忠芳 胡小丽 孔玲 刘绍璞 |
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| 作者单位： | [1]School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China [2]Department of Chemistry and Environment Engineering, Fuling Normal University, Fuling, Chongqing 408000, China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China （No. 20475045）. |
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| 摘 要： | In weak acidic medium, anticancer antibiotics bleomycin A5 （BLMA5） and bleomycin A2 （BLMA2） can react with halofluorescein dyes such as erythrosin （Ery）, eosin Y （EY）, eosin B （EB） and rose bengal （RB） by virtue of electrostatic attraction and hydrophobic force to form the ion-association complexes, which can result in the fading reactions of four halofluorescein dyes. The maximum fading wavelengths of these four dyes were located at 527 nm for Ery, 515 nm for EY, 517 nm for EB and 546 nm for RB, respectively. The decrements of absorbance （AA） were directly proportional to the concentrations of bleomycin in a certain range. A new method for the determination of bleomycins anticancer drugs based on fading reactions of halofluorescein dyes has been developed. The method was not only highly sensitive but also simple and rapid. The molar absorptivities （ε） ranged from 1.5 × 10^5 to 7.5 × 10^5 L·mol^-1·cm^-1. It was applied to determination of the bleomycins in human serum, urine and rabbit serum samples. In this work, the spectral properties and the optimum reaction conditions were investigated. The structure of ion-association complexes and the reaction mechanism were discussed.
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| 关 键 词： | 分光光度法 博来霉素 晕荧光素 反应机理 |
| 修稿时间： | 2006-08-212007-04-20 |
A Highly Sensitive Spectrophotometric Assay of Bleomycins Based on the Fading Reaction of Some Halofluorescein Dyes |
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| LIU Jiang-Tao,LIU Zhong-Fang,HU Xiao-Lia, Kong Ling, LIU Shao-Pu.A Highly Sensitive Spectrophotometric Assay of Bleomycins Based on the Fading Reaction of Some Halofluorescein Dyes[J].Chinese Journal of Chemistry,2007,25(8):1156-1162. |
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| Authors: | LIU Jiang-Tao LIU Zhong-Fang HU Xiao-Lia Kong Ling LIU Shao-Pu |
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| Institution: | 1.School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China;2. Department of Chemistry and Environment Engineering, Fuling Normal University, Fuling, Chongqing 408000, China |
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| Abstract: | In weak acidic medium, anticancer antibiotics bleomycin A5 (BLMA5) and bleomycin A2 (BLMA2) can react with halofluorescein dyes such as erythrosin (Ery), eosin Y (EY), eosin B (EB) and rose bengal (RB) by virtue of electrostatic attraction and hydrophobic force to form the ion‐association complexes, which can result in the fading reactions of four halofluorescein dyes. The maximum fading wavelengths of these four dyes were located at 527 nm for Ery, 515 nm for EY, 517 nm for EB and 546 nm for RB, respectively. The decrements of absorbance ((A) were directly proportional to the concentrations of bleomycin in a certain range. A new method for the determination of bleomycins anticancer drugs based on fading reactions of halofluorescein dyes has been developed. The method was not only highly sensitive but also simple and rapid. The molar absorptivities (ε) ranged from 1.5×105 to 7.5×105 L·mol?1·cm?1. It was applied to determination of the bleomycins in human serum, urine and rabbit serum samples. In this work, the spectral properties and the optimum reaction conditions were investigated. The structure of ion‐association complexes and the reaction mechanism were discussed. |
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| Keywords: | spectrophotometry bleomycin As bleomycin A2 halofluorescein dye |
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