Implementation of Random Bacterial Genomic DNA Microarray Chip (RBGDMC) for Screening of Dominant Bacteria in Complex Cultures |
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Authors: | Byoung Chan Kim Ji Hyun Park Man Bock Gu |
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Institution: | (1) Institut Pasteur Korea, 696 Sampyeong-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400, Republic of Korea;(2) College of Life Sciences and Biotechnology, Korea University, 5-1 Anam-dong, Seongbuk-gu, Seoul, 136-701, Republic of Korea; |
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Abstract: | The random bacterial genomic DNA microarray chip (RBGDMC), which was fabricated using random genomic DNA fragments obtained
from the fragmentation of bacterial genome by using four different pairs of restriction enzymes, was found to discriminate
bacterial species in the same genus and resulted in the determination of dominant bacteria in enriched cultures. The identification
of a dominant bacterial species was successfully conducted in the co-culture of three different bacteria using the RBGDMC.
In addition, the analysis of the chip data could confirm if any of the selected bacteria is the most abundant or if some bacteria
were enriched and became the dominant species within the consortium after the samples were prepared from the repeated cultures
of real sludge in a complex medium. This study shows the successful implementation of the RBGDMC for the identification and
monitoring of dominant bacteria in complex environmental bacterial communities simply without any PCR amplification of the
target nucleic acids. |
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Keywords: | |
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