Development and validation of an LC‐ESI‐MS/MS method for the quantitation of hemslecin A in rhesus monkey plasma and its application in pharmacokinetics |
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| Authors: | Min Bai Hong‐Liang Li Jian‐Chang He Gong‐Hao He En‐Fu Feng Yue‐Qiong Liu Pan‐Pan Shi Gui‐Li Xu |
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| Institution: | 1. Department of Pharmacy, Han Dan Central Hospital, Handan, Hebei, People's Republic of China;2. Institute of Clinical Pharmacology, School of Pharmaceutical Sciences, Sun Yat‐sen University, Guangzhou, China;3. Department of Pharmacy, Kunming General Hospital of Chengdu Military Region, Kunming, People's Republic of China |
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| Abstract: | In this study, a new LC‐ESI‐MS/MS‐based method was validated for the quantitation of hemslecin A in rhesus monkey plasma using otophylloside A as internal standard (IS). Hemslecin A and the IS were extracted from rhesus monkey plasma using liquid–liquid extraction as the sample clean‐up procedure, and were subjected to chromatography on a Phenomenex Luna CN column (150 × 2.0 mm, 3.0 µm) with the mobile phase consisting of methanol and 0.02 mol/mL ammonium acetate (55:45, v/v) at a flow rate of 0.2 mL/min. Detection was performed on an Agilent G6410B tandem mass spectrometer by positive ion electrospray ionization in multiple reaction monitoring mode, monitoring the transitions m/z 580.5 M + NH4]+ → 503.4 and m/z 518.2 M + NH4]+ → 345.0 for hemslecin A and IS, respectively. The assay was linear over the concentration range of 0.5–200 ng/mL and was successfully applied to a pharmacokinetic study in rhesus monkeys. Copyright © 2013 John Wiley & Sons, Ltd. |
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| Keywords: | hemslecin A LC‐ESI‐MS/MS rhesus monkey plasma pharmacokinetics |
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