微流控SERS及其在生物医学应用的研究进展

微流控芯片以其对微量样品的精确操控能力而引起特别关注,表面增强拉曼光谱(SERS)作为一种超灵敏的光谱检测技术近年来在痕量检测上应用广泛。微流控芯片与SERS相结合的系统可对微量生物样品进行无损、快速、高灵敏度且高通量的检测分析,在生物医学领域有巨大的应用潜力,是当前的研究热点之一。本文介绍了微流控SERS系统近年的发展情况,包括微流控芯片的制作加工和流体操控,以及微流控芯片中SERS基底的集成;并重点介绍了近年来SERS微流控芯片系统在生物医学上的应用,如生物分子的检测、细胞分析、药物监测和筛选、疾病诊断,以及在环境和食品健康安全方面的检测应用。

Microfluidic SERS form and its Biomedical Applications

Microfluidic chips have attracted a lot of attention for their precise manipulation of micro-volume samples.Surface-enhanced Raman spectroscopy(SERS)as an ultra-sensitive analytical technique has been widely applied in trace detection in recent years.The combination of microfluidic chip and SERS can provide rapid,non-destructive,high-sensitivity and high-throughput analysis for biological samples,which is of great significance to the development of potential biomedical applications,thus occupying an outstanding position among the current research hot topics.This paper briefly introduces the recent developments of microfluidic SERS systems,including the fabrication of microfluidic chips,the manipulation of fluid and the integration of SERS substrates in microfluidic chips,and in particular attempts to give a comprehensive review on the biomedical applications of microfluidic SERS systems in recent years,including biomolecule detection,cell analysis,drug monitoring and screening,disease diagnosis,environment/food health and safety inspection.