Electrochemical biosensor based on hairpin DNA probe using 2-nitroacridone as electrochemical indicator for detection of DNA species related to Chronic Myelogenous Leukemia |
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| Institution: | 1. Ministry of Education Key Laboratory of Analysis and Detection for Food Safety (Fuzhou University), and Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China;2. Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China;3. Pharmaceutical Department of Fujian College of Medical Occupation and Technology, Fuzhou 350101, China |
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| Abstract: | In this article, a new kind of hairpin DNA Electrochemical biosensor using nitroacridone as electrochemical indicator was first designed, and used to detect BCR/ABL fusion gene in Chronic Myelogenous Leukemia (CML). The results indicated that in pH 7.0 Tris–HCl buffer solution, the oxidation peak current was linear with the concentration of complementary strand in the range of 6.2 × 10?8–3.1 × 10?7 mol/l with a detection limit of 5.3 × 10?9 mol/l. This new hairpin DNA electrochemical biosensor demonstrates its excellent specificity for single-base mismatch and complementary (dsDNA) after hybridization, and this probe has been used for assay of PCR product of a real sample with satisfactory result. |
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