A fast determination of yohimbine in pharmaceuticals by micellar electrokinetic chromatography |
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| Authors: | Kateřina Vítková Markéta Pechová Jan Petr Joanna Znaleziona Václav Ranc Vítězslav Maier Juraj Ševčík |
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| Institution: | 1.Department of Analytical Chemistry, Faculty of Science,Palacky University in Olomouc,Olomouc,Czech Republic;2.Department of Clinical Biochemistry,University Hospital Olomouc,Olomouc,Czech Republic;3.Laboratory of Physicochemistry of Electrolytes,Colloids and Analytical Sciences, UMR CNRS 7195,Paris,France;4.Ecole Nationale Supérieure de Chimie de Paris,Chimie ParisTech,Paris Cedex 05,France |
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| Abstract: | A method for the fast determination of yohimbine, a potent adrenoreceptor antagonist used for the treatment of sexual dysfunctions,
is proposed in this article. MEKC under basic and acidic conditions (sodium borate, pH 9.5 and sodium phosphate, pH 2.5) with
SDS was developed. The effect of the experimental parameters, e.g. pH, SDS concentration and injection time, on yohimbine
migration was also studied. Both methods were validated in terms of linearity, limits of detection and quantification, accuracy,
and precision using caffeine as an internal standard. The application for the determination of yohimbine in hand-made medicaments
is also investigated in this study.
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