Preparation and Evaluation of Poly-l-Lysine Stationary Phase for Hydrophilic Interaction/Reversed-Phase Mixed-Mode Chromatography |
| |
| Authors: | Yuanyuan Li Zhigang Xu Yaoyao Feng Xiaoyan Liu Tong Chen Haixia Zhang |
| |
| Institution: | 1. Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, Lanzhou, 730000, China
2. Key Laboratory of Energy and Chemical Engineering, Ningxia University, Yinchuan, 750021, China
|
| |
| Abstract: | In this work, a poly-l-lysine-grafted stationary phase was synthesized by polymerization of N-carboxyanhydride of l-lysine initiated by 3-aminopropylated silica. The resulting material was characterized by FT-IR spectra, elemental analysis and thermogravimetric analysis, which clearly indicated that the new phase had been prepared successfully. The retention of polar solutes depending on acetonitrile content in mobile phase exhibited ??U-shaped?? curves, which was an indication of hydrophilic interaction liquid chromatography (HILIC)/reversed-phase liquid chromatography (RPLC) mixed-mode retention behavior. The retention mechanisms in HILIC and RPLC modes also were investigated. Phenol compounds, aniline compounds and hydrophilic compounds were separated in RPLC or HILIC mode on the new stationary phase, respectively. This result shows that the new phase could be used for both RPLC and HILIC applications, providing greater flexibility for real sample analysis. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
