Full-field quantitative phase imaging by white-light interferometry with active phase stabilization and its application to biological samples |
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Authors: | Li Xinhong Yamauchi Toyohiko Iwai Hidenao Yamashita Yutaka Zhang Haijun Hiruma Teruo |
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Institution: | State Key Laboratory of Modern Optical Instrumentation, Zhejiang University, Hangzhou 310027, China. |
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Abstract: | We report a Koehler-illumination-based full-field, actively stabilized, low-coherence phase-shifting interferometer, which is built on a white-light Michelson interferometer. By using a phase-stepping technique we can obtain full-field phase images of the sample. An actively stabilized phase-lock circuit is employed in the system to reduce phase noise. An application to human epithelial cells (HeLa cells) is achieved in our experiment. The advancement of this technique rests in its ability to take images of unstained biological samples quantitatively and on a nanometer scale. |
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