| 人源化甲状腺素脱碘酶单链抗体的结构模拟 |
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| 引用本文： | 林凤,章威,陈默,丁兰,刘兰英,徐筱杰,赵大庆,倪嘉缵.人源化甲状腺素脱碘酶单链抗体的结构模拟[J].高等学校化学学报,2002,23(6):1065-1068. |
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| 作者姓名： | 林凤 章威 陈默 丁兰 刘兰英 徐筱杰 赵大庆 倪嘉缵 |
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| 作者单位： | 1. 中国科学院长春应用化学研究所稀土化学与物理开放实验室, 长春 130022;
2. 吉林大学生命科学学院, 长春 130023;
3. 北京大学化学与分子工程学院, 北京 100871 |
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| 基金项目： | 国家重大项目基金 (批准号 :KJ95 1-A1-5 0 4-0 2 )，中国科学院基础局基金 (批准号 :2 970 10 0 5 )，国家杰出青年科学基金(批准号 :2 962 5 2 0 3 )资助 |
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| 摘 要： | 以四碘甲状腺素原氨酸为半抗原,从人噬菌体展示半合成单链抗体库中筛选与半抗原有特异结合性的单链抗体E3,并测定了E3的一级结构,同时用计算机模拟了其空间结构.
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| 关 键 词： | 甲状腺素脱碘酶 单链抗体 计算机模拟 |
| 文章编号： | 0251-0790(2002)06-1065-04 |
| 收稿时间： | 2001-03-02 |
Computer Simulation of Human Iodothyronine Deiodinase Single-chain Antibody |
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| LIN Feng ,ZHANG Wei ,CHEN Mo ,DING Lan ,LIU Lan Ying ,XU Xiao Jie ZHAO Da Qing ,NI Jia Zuan.Computer Simulation of Human Iodothyronine Deiodinase Single-chain Antibody[J].Chemical Research In Chinese Universities,2002,23(6):1065-1068. |
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| Authors: | LIN Feng ZHANG Wei CHEN Mo DING Lan LIU Lan Ying XU Xiao Jie ZHAO Da Qing NI Jia Zuan |
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| Institution: | 1. Laboratory of Rare Earth Chemistry and Physics, Changchun Insitute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2. College of Life Science, Jilin University, Chang chun 130023, China;
3. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China |
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| Abstract: | Iodothyronine plays a major role in growth, basic metabolism and organ formation. It has an extremely limited source in the body. In this thesis, we designed iodothyronine(T4) as hapten. Then a single chain antibody displayed on phange was obtained from a human phage displaying a single chain antibody library. The specific genes of E3 was subcloned in P 5E vector. According to its amino acid sequences, we simulate its three dimention structure by computer. It has never been reported in PDB. |
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| Keywords: | Iodothyronine deiodinase Single chain antibody Computer simulation |
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