Interaction of the flavonoid hesperidin with bovine serum albumin: A fluorescence quenching study |
| |
Authors: | Yan-Qing Wang Hong-Mei Zhang Wei-Hua Tao |
| |
Institution: | a Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Normal College, Yancheng City, Jiangsu Province 224002, People's Republic of China b Institute of Applied Chemistry and Environmental Engineering, Yancheng Normal College, Yancheng City, Jiangsu Province 224002, People's Republic of China |
| |
Abstract: | The interaction between the flavonoid hesperidin and bovine serum albumin (BSA) was investigated by fluorescence and UV/Vis absorption spectroscopy. The results revealed that hesperidin caused the fluorescence quenching of BSA through a static quenching procedure. The hydrophobic and electrostatic interactions play a major role in stabilizing the complex. The binding site number n, and apparent binding constant KA, corresponding thermodynamic parameters ΔGo, ΔHo, ΔSo at different temperatures were calculated. The distance r between donor (BSA) and acceptor (hesperidin) was obtained according to fluorescence resonance energy transfer. The effect of Cu2+, Zn2+, Ni2+, Co2+, and Mn2+ on the binding constants between hesperidin and BSA were studied. The effect of hesperidin on the conformation of BSA was analyzed using synchronous fluorescence spectroscopy and UV/Vis absorption spectroscopy. |
| |
Keywords: | Hesperidin Bovine serum albumin Intrinsic fluorescence Binding thermodynamics Fluorescence resonance transfer |
本文献已被 ScienceDirect 等数据库收录! |
|