Oxidized multiwalled carbon nanotubes for quantitative determination of cationic surfactants in water samples using atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry |
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Authors: | Shrivas Kamlesh Wu Hui-Fen |
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Institution: | a IMAGES EA 4218, Centre de phytopharmacie, Université de PerpignanVia Domitia, Perpignan Cedex, France b Equipe de Recherche Electrochimie et Systèmes Interfaciaux, Faculté des Sciences de Tétouan, Université Abdelmalek Essaâdi. B.P. 2121, 93000, Tétouan- Morocco c Department of Analytical Chemistry, Faculty of Sciences, University of Cádiz, Spain d Department of Chemistry, Fudan University, Shanghai 200433, China |
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Abstract: | A new biosensor for detection of phenols, based on tyrosinase immobilization with alumina sol-gel on Sonogel-Carbon transducer, has been developed. The electrode was prepared using high energy ultrasounds directly applied to the precursors. The alumina sol-gel provided a microenvironment for retaining the native structure and activity of the entrapped enzyme and a very low mass transport barrier to the enzyme substrates. Phenols are oxidized by tyrosinase biosensor to form a detectable product, which was determined at −300 mV vs. Ag/AgCl reference electrode. For phenol, the sensor exhibited a fast response which resulted from the porous structure and high enzyme loading of the sol-gel matrix. The linear range was from 5 × 10−7 M to 3 × 10−5 M, with a detection limit of 3 × 10−7 M. The stability of the biosensor was also evaluated. |
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Keywords: | Biosensors Sonogel-Carbon electrode Tyrosinase Alumina sol-gel Phenolic compounds |
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