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| 三维荧光光谱二阶校正方法测定血浆样中喷昔洛韦及泛昔洛韦含量 | |
| 引用本文： | 宋晶晶,吴海龙,于永杰,黄蕾,俞汝勤. 三维荧光光谱二阶校正方法测定血浆样中喷昔洛韦及泛昔洛韦含量[J]. 中国科学:化学, 2010, 0(8): 1136-1141 |
| 作者姓名： | 宋晶晶 吴海龙 于永杰 黄蕾 俞汝勤 |
| 作者单位： | 化学生物传感与计量学国家重点实验室；湖南大学化学化工学院,长沙410082 |
| 基金项目： | 国家自然科学基金（20775025）; 教育部长江学者和创新团队发展计划基金资助 |
| 摘 要： | 利用三维荧光光谱即激发发射矩阵荧光光谱与化学计量学的基于平行因子分析(PARAFAC)算法的二阶校正方法相结合,尝试对血浆样中泛昔洛韦及其活性代谢物喷昔洛韦的含量进行同时定量测定.当算法选取组分数为3时,解析得到血浆样中泛昔洛韦和喷昔洛韦的平均回收率分别为(102.4±3.4)%和(105.1±2.3)%.结果表明该分析策略可准确可靠地实现血浆样中泛昔洛韦和喷昔洛韦的直接同时快速定量测定. |
| 关 键 词： | 二阶校正 平行因子分析算法 激发发射矩阵荧光光谱 喷昔洛韦 泛昔洛韦 血浆样 |
Determination of Famciclovir and Penciclovir in human plasma samples by using excitation-emission matrix fluorescent spectra coupled with second-order calibration |
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| SONG JingJing,WU HaiLong,YU YongJie,HUANG Lei,YU RuQin. Determination of Famciclovir and Penciclovir in human plasma samples by using excitation-emission matrix fluorescent spectra coupled with second-order calibration[J]. Scientia Sinica Chimica, 2010, 0(8): 1136-1141 | |
| Authors: | SONG JingJing WU HaiLong YU YongJie HUANG Lei YU RuQin |
| Affiliation: | SONG JingJing,WU HaiLong,YU YongJie,HUANG Lei, YU RuQin |
| Abstract: | Famciclovir and Pencilovir contents in human plasma samples have been tried to determine simultaneously by using second-order calibration method based on Parallel Factor Analysis （FAFARAC） algorithm combined with excitation-emission matrix fluorescent spectra. When the component number in the case was set to 3,the average recoveries of Famciclovir and Penciclovir in human plasma samples were （102.4±3.4）% and （105.1±2.3）% ,respectively. The satisfactory results showed that the second-order calibration method can be applied to quantify Famciclovir and Penciclovir in human plasma samples. |
| Keywords: | second-order calibration Parallel Factor Analysis algorithm excitation-emission fluorescent spectra Penciclovir Famciclovir plasma samples |
| 本文献已被 CNKI 维普 等数据库收录！ | |