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| Capillary electrophoretic separation of glycoproteins | |
| 引用本文： | 陈义. Capillary electrophoretic separation of glycoproteins[J]. 中国科学B辑(英文版), 1998, 41(1): 71-76. DOI: 10.1007/BF02875560 |
| 作者姓名： | 陈义 |
| 作者单位： | Institute of Chemistry，Chinese Academy of Sciences，Beijing 100080，China |
| 基金项目： | ProjectsupportedbytheNationalNaturalScienceFoundationofChina (GrantNo .2 9835 0 2 0 )bytheChineseAcademyofSciences(CAS)andbytheDirectorFundsoftheInstituteofChemistryoftheCAS |
| 摘 要： | Ｇｌｙｃｏｐｒｏｔｅｉｎｓａｒｅｋｎｏｗｎｔｏｂｅａｎｉｍｐｏｒｔａｎｔｇｒｏｕｐｏｆｆｕｎｃｔｉｏｎａｌｍａｃｒｏｍｏｌｅｃｕｌｅｓｉｎｂｉｏｌｏｇｉｃａｌｓｙｓｔｅｍｓ,ｂｕｔｉｔｉｓｍｏｒｅｄｉｆｆｉｃｕｌｔｔｏａｎａｌｙｚｅｔｈｅｍｔｈａｎｎｏｒｍａｌｐｒｏｔｅｉｎｓ.Ｔｈｅｍｏｓｔｓｅｒｉｏｕｓｐｒｏｂｌｅｍｌｉｅｓｉｎｔｈｅａｎａｌｙｓｉｓｏｆｔｈｅｉｒｍｉｃｒｏｈｅｔｅｒｏｇｅｎｅｉｔｙｗｈｉｃｈｉｓａｓｐｅｃｉａｌｐｈｅｎｏｍｅｎｏｎｏｆｇｌｙｃｏｃｏｎｊｕｇａｔｅｓ.Ｄｉｆｆｅｒ… |
| 收稿时间： | 1997-06-23 |
Capillary electrophoretic separation of glycoproteins |
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| Yi Chen. Capillary electrophoretic separation of glycoproteins[J]. Science in China(Chemistry), 1998, 41(1): 71-76. DOI: 10.1007/BF02875560 | |
| Authors: | Yi Chen |
| Affiliation: | (1) Institute of Chemistry, Chinese Academy of Sciences, 100080 Beijing, China |
| Abstract: | A capillary electrophoretic approach has been developed for analyzing the microheterogeneity of glycoproteins. The key factors include the buffer composition, concentration, pH, and the surface treatment of the capillaries used. It has been shown that, if the buffer is mainly composed of alcohol amines and the capillaries are coated with polyacrylamide, ovalbumin from chicken egg can be separated into more than 25 peaks and that of transferrin about 10 peaks. |
| Keywords: | glycoprotein microheterogeneity capillary electrophoresis. |
| 本文献已被 CNKI SpringerLink 等数据库收录！ | |