| 一种用于细胞核成像的新型双光子荧光探针 |
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| 引用本文： | 刘鑫,刘恒,贾鹏飞,张波,王军杰,赵宁,张元红,于晓强.一种用于细胞核成像的新型双光子荧光探针[J].高等学校化学学报,2009,30(3):465-467. |
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| 作者姓名： | 刘鑫 刘恒 贾鹏飞 张波 王军杰 赵宁 张元红 于晓强 |
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| 作者单位： | 1. 兰州大学生命科学学院,兰州,730000
2. 山东大学晶体材料国家重点实验室,济南,250100 |
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| 摘 要： | 本文报道了具有大双光子活性吸收截面的DNA探针BMVEC, 同时首次利用双光子显微镜完成了与DAPI的复染实验, 实验结果证实了BMVEC对细胞核的选择性标记能力.
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| 关 键 词： | 双光子荧光探针 双光子荧光成像 核酸 |
| 收稿时间： | 2008-12-02 |
New Two-photon Fluorescence Probe for Nuclear DNA Imaging |
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| LIU Xin,LIU Heng,JIA Peng-Fei,ZHANG Bo,WANG Jun-Jie,ZHAO Ning,ZHANG Yuan-Hong,YU Xiao-Qiang.New Two-photon Fluorescence Probe for Nuclear DNA Imaging[J].Chemical Research In Chinese Universities,2009,30(3):465-467. |
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| Authors: | LIU Xin LIU Heng JIA Peng-Fei ZHANG Bo WANG Jun-Jie ZHAO Ning ZHANG Yuan-Hong YU Xiao-Qiang |
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| Institution: | 1.School of Life Science;Lanzhou University;Lanzhou 730000;China;2.State Key Lab of Crystal Materials;Shandong University;Jinan 250100;China |
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| Abstract: | A new two-photon fluorescence DNA probe, BMVEC, with bigger σ×Φ(1437 GM) was synthesized. The absorption titration shows that there is an interaction between BMVEC and DNA because the probe can intercalate the grooves in the double helix of DNA, which enhances its two-photon fluorescent intensity. Meanwhile the double-staining results in cancer cells by means of TPM demonstrate the exclusive nucleic labeling ability of BMVEC, and the positions and amounts of nuclei labeled by BMVEC are wholly the identical as those by DAPI. Moreover, the incident power of BMVEC is lower than that of DAPI. The results indicate that BMVEC can act as the two-photon fluorescent probe of DNA. |
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| Keywords: | Two-photon fluorescent probe Two-photon fluorescence imaging Nucleic acid |
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