DNA biosensor based on the electrochemiluminescence of Ru(bpy)3(2+) with DNA-binding intercalators |
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Authors: | Lee Jeong-Gun Yun Kyusik Lim Guei-Sam Lee Sang Eun Kim Suhyeon Park Je-Kyun |
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Institution: | Bio Electronics Group, LG Electronics Institute of Technology, 16 Woomyeon-dong, Seocho-gu, Seoul 137-724, South Korea. |
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Abstract: | This paper reports a novel detection method for DNA hybridization based on the electrochemiluminescence (ECL) of Ru(bpy)(3)(2+) with a DNA-binding intercalator as a reductant of Ru(bpy)(3)(3+). Some ECL-inducible intercalators have been screened in this study using electrochemical methods combined with a chemiluminescent technique. The double-stranded DNA intercalated by doxorubicin, daunorubicin, or 4',6-diamidino-2-phenylindole (DAPI) shows a good ECL with Ru(bpy)(3)(2+) at +1.19 V (versus Ag/AgCl), while the non-intercalated single-stranded DNA does not. In order to stabilize the self-assembled DNA molecules during ECL reaction, we constructed the ECL DNA biosensor separating the ECL working electrode with an immobilized DNA probe. A gold electrode array on a plastic plate was assembled with a thru-hole array where oligonucleotide probes were immobilized in the side wall of thru-hole array. The fabricated ECL DNA biosensor was used to detect several pathogens using ECL technique. A good specificity of single point mutations for hepatitis disease was obtained by using the DAPI-intercalated Ru(bpy)(3)(2+) ECL. |
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Keywords: | Electrochemiluminescence (ECL) Tris-(2 2′ -bipyridyl)ruthenium(II) DNA-binding intercalator 4′ 6-diamidino-2-phenylindole (DAPI) DNA biosensor DNA hybridization detection |
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