| 新型乳酸固定化酶荧光毛细生物传感器 |
| |
| 引用本文： | 李永生,鞠香,高秀峰,杨微. 新型乳酸固定化酶荧光毛细生物传感器[J]. 分析化学, 2009, 37(5) |
| |
| 作者姓名： | 李永生 鞠香 高秀峰 杨微 |
| |
| 作者单位： | 1. 四川大学化学工程学院,成都,610065
2. 四川大学基础医学与法医学院,成都,610065 |
| |
| 基金项目： | 四川大学214振兴计划 |
| |
| 摘 要： | 对长45 mm、内径0.9 mm的医用毛细管进行γ-氨丙基三乙氧基硅烷氨基化和戊二醛醛基化后,再将乳酸脱氢酶(LDH)的氨基与戊二醛的醛基结合,使其固定在毛细管内壁,构成一种新型固定化酶乳酸荧光毛细生物传感器(IE-LFCBS),实现了对乳酸的微量、快速测定.IE-LFCBS吸入辅酶Ⅰ与乳酸的混合液,在固定化酶催化下使乳酸与辅酶Ⅰ反应,生成荧光物质还原型辅酶Ⅰ;激发波长353 nm、发射波长466 nm.适用于IE-LFCBS的优化条件为:辅酶Ⅰ浓度4 mmol/L、用于固定化的LDH浓度60 kU/L、反应时间15 min、反应温度38 ℃、测定范围为1.0～5.0 mmol/L、回收率95%～98%,IE-LFCBS的相对标准偏差为RSD<1.5%(n=11),检出限为0.45 mmol/L.IE-LFCBS的试液用量极少(18 μL),并能重复使用,可望用于发酵食品、药品、血液标本等各类样品中乳酸的快速检测.
|
| 关 键 词： | 固定化酶 荧光毛细分析 还原型辅酶Ⅰ 乳酸 生物传感器 |
A Novel Immobilization Enzyme Lactate Fluorescence Capillary Biosensor |
| |
| LI Yong-Sheng,JU Xiang,GAO Xiu-Feng,YANG Wei. A Novel Immobilization Enzyme Lactate Fluorescence Capillary Biosensor[J]. Chinese Journal of Analytical Chemistry, 2009, 37(5) |
| |
| Authors: | LI Yong-Sheng JU Xiang GAO Xiu-Feng YANG Wei |
| |
| Affiliation: | School of Chemical Engineering;Sichuan University;Chengdu 610065;West China School of Preclinical and Forensic Medicine;Chengdu 610065 |
| |
| Abstract: | Lactate dehydrogenase(LDH) was immobilized in inner surface of a medical capillary having 45 mm of length and 0.9 mm of inner diameter,and an immobilization enzyme lactate fluorescence capillary biosensor(IE-LFCBS) was made.Rapid microanalysis for lactate was realized by using it.When the IE-LFCBS sucked the mixture solution of coenzyme I(NAD+) and lactate in it,under catalysis of immobilized lactate dehydrogenase,NAD+ reacted with lactate to form NADH,and then fluorescence intensity of NADH in the IE-LFCBS... |
| |
| Keywords: | |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
