对痕量羊角材料降解产物的表面增强拉曼光谱研究

将一定浓度的硝酸银与柠檬酸三钠混合后，利用微波加热法制备纳米银溶胶，该方法加热速度快、温度分布均匀、反应条件易控制。采用准弹性激光散射技术检测其粒度大小及分布状态的信息，测得其平均粒度为(53.27±2.65)nm，粒度大小集中分布在56 nm左右。然后以之作为表面增强拉曼光谱(SERS)的活性基底，研究测定羊角痕量降解产物的SERS光谱。结果发现，羊角降解产物的表面增强拉曼效应显著，尤其在659，830，850，929，999，1 028，1 280，1 439，1 599 cm-1等多处出现了明显的拉曼振动峰，这些谱峰反映了羊角降解产物的生化成分信息。并且通过对所获得的羊角降解产物SERS信号进行的谱峰归属分析表明，羊角降解产物主要为氨基酸与多肽类物质。运用表面增强拉曼光谱检测角材料的降解产物，获得较高的灵敏度，可测定浓度低至ppm水平的痕量降解产物，本研究表明，SERS可能为羊角等角材料降解产物的检测提供一种快速、直观、准确的新方法。

Surface-Enhanced Raman Spectra Analysis of Trace Degradation Products from Goat Horn

Nano-silver colloid was synthesized by using microwave method on the mixtures of sodium citrate solution and silver nitrate solution. The method has advantages of fast heating speed, uniform temperature distribution and easily controlled reaction conditions. The sizes and size distributions of the silver particles were characterized by means of quasi-elastic laser scattering (QLS). The average particles size was (53.27±2.65) nm and the size of the particles was mainly distributed around 56 nm. Surface-enhanced Raman spectra of the degradation products from goat horn were obtained with silver colloid as active substrate. It was observed that the Raman signal of SERS was enhanced significantly compared with that of regular Raman spectrum, especially at the Raman bands of 659, 830, 850, 929, 999, 1 028, 1 280, 1 439 and 1 599 cm-1 which reflect the biochemical components in degradation products. The characteristic Raman bands of degradation products from goat horn were preliminary assigned. The assignments showed that the main constituents of the degradation products from goat horn were amino acids and polypeptides. It was for the first time that Surface-enhanced Raman spectroscopy was used to detect trace degradation products from the horns. Raman signal enhancement can be obtained with high sensitivity for the trace concentrations as low as ppm level. It is concluded that surface-enhanced Raman spectroscopy can provide a fast, direct and precise detecting method for the detection of trace degradation solution from horns.