Recognition of solvent exposed protein surfaces using anthracene derived receptors |
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Authors: | Wilson Andrew J Hong Jason Fletcher Steven Hamilton Andrew D |
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Institution: | Department of Chemistry, PO Box 208107, 225 Prospect Street, Yale University, New Haven, CT 06520-8107, USA. |
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Abstract: | A new class of receptor is described that can selectively bind to the solvent exposed surface of proteins such as cytochrome c and lysozyme with low micromolar affinity over cytochrome c551, alpha-lactalbumin, myoglobin and RNase A, under physiologically relevant conditions (5 mM phosphate, pH 7.4). The use of anthracene as a hydrophobic scaffold allows the receptor to act as a selective chemosensor via fluorescence quenching or FRET. The study reveals that co-operative electrostatic interactions over a large surface area dominate binding. Further investigations reveal that the receptor binds to the solvent exposed heme edge of cytochrome c inhibiting its reaction with small reducing agents and validating the strategy for the disruption of protein function. |
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