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Decreased l-tryptophan concentration in distinctive brain regions of mice treated repeatedly with phencyclidine |
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| Authors: | Hideaki Iizuka Shoko Watanabe Mayuko Koshikawa Yurie Matsumoto Yuki Aoyama Hideaki Ichiba Toshitaka Nabeshima Takeshi Fukushima |
| Institution: | 1. Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi-shi, Chiba, 274-8510, Japan 2. Department of Chemical Pharmacology, Meijo University Graduate School of Pharmaceutical Sciences, Nagoya, 468-8503, Japan 3. Department of Psychiatry, Nagoya University Graduate School of Medicine, Nagoya, 464-8601, Japan 4. Academic Frontier Project for Private University, Comparative Cognitive Science Institution, Meijo University, Nagoya, 468-8503, Japan 5. Department of Regional Pharmaceutical Care and Science, Meijo University, Nagoya, 468-8503, Japan |
| Abstract: | It has been reported that repeated phencyclidine (PCP) treatment induces schizophrenia-like behavior in mice. l-Tryptophan (Trp) concentrations in brain tissues of control (n?=?8) and PCP-treated mice (10 mg/kg/day, s.c., 14 days, n?=?10) were determined using high-performance liquid chromatography (HPLC) with fluorescence detection. The HPLC method involved pre-column fluorescence derivatization with (R)-(?)-4-(N,N-dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole (DBD-PyNCS). Eight different parts of the brain, namely, the frontal cortex, nucleus accumbens, striatum, hippocampus, amygdala, thalamus, hypothalamus, and cerebellum, of both groups were investigated. A significant decrease in the l-Trp concentration in the nucleus accumbens (p?=?0.024) and hippocampus (p?=?0.027) was observed in PCP-treated mice, suggesting that alteration of the l-Trp metabolism might occur in these brain parts. |
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