| 运用预饱和对HOESY实验灵敏度优化 |
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| 引用本文： | 姚雪军,朱广.运用预饱和对HOESY实验灵敏度优化[J].波谱学杂志,2008,25(4):447-452. |
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| 作者姓名： | 姚雪军 朱广 |
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| 作者单位： | （1.上海市功能磁共振成像重点实验室，华东师范大学 物理系, 上海 200062； ;2.香港科技大学 生物化学系，香港 ） ; |
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| 摘 要： | 二维异核NOESY(HOESY)内在的低灵敏度限制其在结构分析上的应用，利用1H探测（反式探测）可以提高其灵敏度. 这里我们提出一种预饱和二维13C-1H HOESY实验，利用异核之间的NOE增强效应，进一步提高实验的灵敏度. 1H探测HOESY谱图中经常有严重的t1噪声，可以通过脉冲梯度场相关选择和相位循环压制，得到改善的13C-1H的NOE相关谱图.
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| 关 键 词： | NMR 异核NOESY 预饱和 腺苷酸 灵敏度增强 |
| 收稿时间： | 2008-1-10 |
| 修稿时间： | 2008-3-13 |
Sensitivity Improvement by Presaturation in 2D HOESY |
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| YAO Xue-jun,ZHU Guang.Sensitivity Improvement by Presaturation in 2D HOESY[J].Chinese Journal of Magnetic Resonance,2008,25(4):447-452. |
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| Authors: | YAO Xue-jun ZHU Guang |
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| Institution: | （1.Shanghai Key Laboratory of Functional Magnetic Resonance Imaging, ;Department of Physics， East China Normal University, Shanghai 200062, China; 2.Department of Biochemistry, Hong Kong University of Science and Technology, Hong Kong, China） ; |
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| Abstract: | The low intrinsic sensitivity of 2D heteronuclear NOE (HOESY) experiment has hampered its applications in structural analysis. The sensitivity of the HOESY experiment can be improved by 1H detection (inversed detection). Here we present a 2D 13C-1H HOESY experiment with presaturation to further improve its sensitivity by NOE enhancement. In the proposed experiment, t1 noises in the 1H detected HOESY spectra are suppressed by pulse field gradient (PFG) coherence selection and phase cycles. It was demonstrated that 13C-1H NOE correlation spectra with improved sensitivity could be obtained with the proposed experiment. |
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| Keywords: | NMR |
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