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MCM-41 mesoporous material modified carbon paste electrode for the determination of cardiac troponin I by anodic stripping voltammetry |
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| Authors: | Guo Huishi He Nongyue Ge Shuxun Yang Di Zhang Jinan |
| Affiliation: | a State Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing 210096, China b Institute of Cardiovascular Disease, Nanjing Medical University, Nanjing 210029, China c Laboratory of NanoBio Medicines and Devices, Zhuzhou Institute of Technology, Zhuzhou 412008, China |
| Abstract: | An anodic stripping voltammetric method for the determination of cardiac troponin I (cTnI) at a MCM-41 mesoporous material modified carbon paste electrode (MCM-MCPE) was investigated. The test was based on the dual monoclonal antibody “sandwich” principle using colloidal gold as a labeled substrate. Four main steps were carried out to obtain the analytical signal, i.e. electrode preparation, immunoreaction, silver enhancement, and anodic stripping voltammetric detection. The anodic stripping peak current increased linearly with the concentration of cTnI over the range of 0.8-5.0 ng/ml. A detection limit of 0.5 ng/ml was obtained. The established method was applied to detect cTnI in acute myocardial infarction (AMI) samples using routine enzyme-linked immunoadsorbent assay (ELISA) for comparison analysis, and good results were obtained. |
| Keywords: | Cardiac troponin I Mesoporous materials MCM-41 Carbon paste electrode Anodic stripping voltammetry |
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