Screening for exogenous androgen anabolic steroids in human hair by liquid chromatography/orbitrap-high resolution mass spectrometry |
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| Authors: | Sabina Strano-Rossi,Erika Castrignanò ,Luca Anzillotti,Sara Odoardi,Fabio De-Giorgio,Ana Bermejo,Vincenzo L. Pascali |
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| Affiliation: | 1. Institute of Legal Medicine, Catholic University of Sacred Heart, L.go F. Vito, 1, 00168 Rome, Italy;2. Institute of Legal Medicine, University of Santiago de Compostela, Av S. Francisco s/n, Santiago de Compostela, Spain |
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| Abstract: | A method for the screening of various anabolic steroids and their esters in human hair, based on liquid-chromatography–high resolution mass spectrometry using an Exactive benchtop Orbitrap mass spectrometer, has been set up and validated. This method involved methanolic incubation of 30 mg of hair and analysis of the relevant extract in HPLC using a C18 column. The mass detector, with nominal resolving power of 100,000, operated in full scan mode in APCI under positive ionization mode. Analytes were identified by exact mass, correspondence of isotopic cluster and retention times. |
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| Keywords: | Anabolic steroids Esters Hair analysis High resolution mass spectrometry Orbitrap Forensic toxicology |
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