| 漆树漆酶催化2,6-二甲基酚的氧化反应 |
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| 引用本文： | 季立才,胡培植,徐文中.漆树漆酶催化2,6-二甲基酚的氧化反应[J].高等学校化学学报,1992,13(7):940-944. |
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| 作者姓名： | 季立才 胡培植 徐文中 |
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| 作者单位： | 武汉大学化学系, 武汉, 430072 |
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| 摘 要： | 在0.1mol/L磷酸盐缓冲液中,漆树漆酶催化2,6-二甲基酚(DMP)氧化生成3,3'5.5'-四甲基-4,4'-二苯醌(TMDQ).pH8.0,温度40～50℃时酶促氧化DMP较快.DMP浓度在1.66×10-4～9.92×10-4mol/L时为一级反应,速率常数为3.57×10-5s-1,共存的二茂铁(Fc-H)能大大促进酶促氧化DMP的速度.提出了该反应可能的反应机理。
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| 关 键 词： | 漆树漆酶 2 6-二甲基酚 二茂铁 酶促氧化 3 3'5 5'-四甲基-4 4'-二苯醌 |
| 收稿时间： | 1991-07-15 |
Rhus Vernicifera Laccase Catalyzes Oxidation of 2,6-Dimethylphenol |
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| JI Li-cai,HU Pei-zhi,XU Wen-zhong.Rhus Vernicifera Laccase Catalyzes Oxidation of 2,6-Dimethylphenol[J].Chemical Research In Chinese Universities,1992,13(7):940-944. |
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| Authors: | JI Li-cai HU Pei-zhi XU Wen-zhong |
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| Institution: | Department of Chemistry, Wuhan University, Wuhan, 430072 |
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| Abstract: | Rhus vernicifera laccase catalyzes the oxidation of 2, 6-dimethylphenol(DMP) to 3, 3', 5,5' -tetramethyl-4,4' -diphenoquinone (TMDQ) in 0.1 rnol/Lphosphate buffer solution.The enzymatic oxidation of DMPis much faster at pH 8.0 and temperature 40～50℃.The reaction is a first-order and the rate constant (kobs) is 3.57× 10-5 s-1 when DMP concentration is in the range of 1.66 × 10-4～9.92 × 10-4 mol/L.The coexistent ferrocene (Fc-H) can more greatly promote the enzymatic oxidation of DMP.The possible mechanism of laccase-catalyzed oxidation of DMPand coexistent Fc-H was inferred. |
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| Keywords: | Rhus vernicifera laccase 2 6-Dimethylphenol Ferrocene Enzymatic promation oxidation 3 3' 5 5'-Tetramethyl-4 4'-diphenoquinone |
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