A Highly Selective Fluorescent Probe for Detection of Biological Samples Thiol and Its Application in Living Cells |
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| Authors: | Qing-Ping Zuo Bing Li Qi Pei Zuojun Li Shi-Kun Liu |
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| Affiliation: | (1) Department of Pharmacy, The Third Xiangya Hospital, Central South University, Changsha, Hunan, 410013, People’s Republic of China;(2) Pharmaceutics Department, College of pharmacy, Central South University, Changsha, Hunan, 410013, People’s Republic of China;(3) Pharmaceutics Department, College of pharmacy, Central South University, Changsha, Hunan, 410013, People’s Republic of China;(4) Department of Pharmacy, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China; |
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| Abstract: | Probe 1 was designed and synthesized as a new fluorescent molecular probe for thiols in PBS buffer at physiological condition. This fluorescent molecular probe consists of a thiol reaction moiety bound to a coumarin fluorophore. Its fluorescence quantum yield is low, but a drastic enhancement of fluorescence intensity was observed in the presence of thiols. Possible interference with other analytes was examined. Probe 1 displays a highly selective fluorescent enhancement with thiols, and the probe was successfully applied to thiols determination in intracellular, in human urine and blood samples. |
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