基于去保护机理的新型H2O2荧光探针的制备及应用

合成的3',6'-对戊酰硼荧光素是一种基于去保护机理的高灵敏H₂O₂荧光增强化合物, 能灵敏地检测出样品中H₂O₂的含量. 用IR, ¹H NMR 对该探针的结构进行了表征, 并且讨论了反应时间、溶剂、碱对探针合成的影响. 研究了探针的最佳响应时间, 结果表明检测过程中反应的最佳时间为3 min. 该探针在2.3×10^－12～2.0×10^－8 mol/L H₂O₂溶液范围内呈荧光增强趋势, 检测下限为2.3×10^－12 mol/L. 此探针重现性良好, 在血清中检测H₂O₂的回收率在93.9%～102.2%之间, 结果令人满意, 具有一定的实用价值.

Synthesis and Application of Novel H2O2 Fluorescent Probe Based on the Deprotection Mechanism

The compound, 3',6'-bis(tetramethylethylenedioxyboryl)fluorane, synthesized in this experiment is a kind of H₂O₂ fluorescence-strengthened reagent, which depends on deprotection mechanism and can examine the H₂O₂ concentration sensitively. The structure of the compound was confirmed by the IR and ¹H NMR spectra. The influences of reaction time, solvent and base on the probe synthesis were investigated in detail. The response time of the fluorescent probe was also studied and as a result, the optimal time for the H₂O₂ detection is 3 min. The fluorescence intensity of the probe was gradually increased with the increase of H₂O₂ concentration in the range of 2.3×10^－12～2.0×10^－8 mol/L, the detection limit of H₂O₂ was 2.3×10^－12 mol/L, and the recovery rates in the blood serum were in the range of 93.9%～102.2%.