| 光谱法研究Ge-132与DNA的作用机理 |
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| 引用本文： | 李文友,朱守田,何锡文,梁宏.光谱法研究Ge-132与DNA的作用机理[J].化学学报,2002,60(1):105-108. |
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| 作者姓名： | 李文友 朱守田 何锡文 梁宏 |
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| 作者单位： | 南开大学化学系;广西师范大学化学与生命科学学院 |
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| 基金项目： | 南开大学元素有机化学国家重点开放实验室基金，广西壮族自治区教育厅科研基金资助项目. |
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| 摘 要： | 利用吸收光谱、DNA碱变性曲线、荧光光谱研究了Ge-132与DNA的相互作用。在Ge-132存在下，DNA的紫外吸收光谱产生明显的减色效应。同时，Ge-132的存在使DNA碱变性的pH值增大，变性后增色效应减小，实验结果表明，Ge-132主要是以嵌入方式与DNA结合的。
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| 关 键 词： | 吸收光谱法 脱氧核糖核酸 有机锗化合物 嵌入反应 抗癌药 药物设计 |
| 修稿时间： | 2001年9月10日 |
Study on the interaction mechanism between Ge-132 and DNA by spectral methods |
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| LI,Wen-You ZHU,Shou-Tian HE,Xi-Wen LIANG,Hong.Study on the interaction mechanism between Ge-132 and DNA by spectral methods[J].Acta Chimica Sinica,2002,60(1):105-108. |
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| Authors: | LI Wen-You ZHU Shou-Tian HE Xi-Wen LIANG Hong |
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| Institution: | LI,Wen-You ZHU,Shou-Tian HE,Xi-Wen LIANG,HongDepartment of Chemistry,Nankai University,Tianjin 300071 College of Chemistry and,Life Science,Guangxi Normal University,Guilin 541004 |
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| Abstract: | The interaction between Ge-132 and DNA was investigated using absorption spectra, DNA alkaline denaturation and fluorescence spectra. Based on the measurements of A260 in different pH media, the alkaline denaturation curves of CT DNA in the absence of and in the presence of Ge-132 were obtained. The results showed that the alkaline denaturation for Ge-132-DNA complex occurred at a higher pH than for DNA alone and the hyperchromicity of Ge-132-DNA complex was smaller than that of DNA, which suggested the intercalation mechanism of Ge-132 into DNA bases. All the exprimental results indicated that the intercalative binding was the major mode for the interaction of Ge-132 with DNA. |
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| Keywords: | Ge-132 DNA intercalative binding |
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