固相微萃取-气相色谱-质谱技术分析槐花的挥发性成分

用顶空固相微萃取操作,结合气相色谱-质谱联用技术对槐花挥发性成分进行了鉴定,并用面积归一化法测定其相对含量.共鉴定出化合物31种,包括酮、醇、酸、甾醇、酚、醛、酯、烷烃、烯烃及杂环等10类化合物,其中酮类化合物占总色谱馏出峰面积的21.15%;醇类化合物占13.78%;酸类占9.15%.其主要化合物有:1,9,12三氧-4,6-二氨基环十四烷-5-硫酮(20.58%),棕榈酸(9.05%),9,12,15-十八三烯醇(7.05%),(Z,Z)-9,12-十八碳二烯酸(6.85%),β-谷甾醇(6.11%).该技术可以简便快捷准确地进行槐花挥发性成分的分析鉴定.

Analysis of the Volatile Compounds in Flos Sophaoae by Solid-phase Microextraction and GC-MS

The volatile components in Flos Sophaoae were obtained by solid-phase microextraction(SPME),and were separated and identified by gas chromatography-mass spectrometer(GC-MS).31 compounds were identified.The main constituents of the extract from Flos Sophaoae were found to be ketones(21.15%),alcohols(13.78%),acids(9.15%),sterols,alkanes,aldehydes,alkanes and heterocyclic compounds.The main compounds were: 1,9,12-trioxa-4,6-diazacyclotetradecane-5-thione(20.58%),hexadecanoic acid(9.05%),9,12,15-octadecatrien-1-ol(7.05%),(Z,Z)9,12-octadecadienic acid(6.85%),β-sitosterol(6.11%).SPME-GC-MS is convenient,rapid and reliable for analysis of volatile components in Flos Sophaoae.