Determination of mono- and dichloroacetic acids in betaine media by liquid chromatography |
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Authors: | Ghassempour Alireza Chalavi Soheila Abdollahpour Assem Mirkhani Seyed Amir |
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Institution: | a Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran, Iran b Department of Chemistry, Faculty of Science, Shahid Beheshti University, Evin, Tehran, Iran c Asalib Company, Semnan Industrial City, Semnan, Iran |
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Abstract: | A simple and sensitive method has been developed for the analysis of residue amounts of chloroacetic acids in betaine samples based on derivatization by 1-naphthylamine (NA). The derivatized compounds are analyzed by reverse phase high performance liquid chromatography using methanol and water as mobile phase in the ratio of 32/68 (v/v) and phenyl column and PDA detection at 222 nm. The detection limits (LOD) of monochloroacetic acid (MCA) and dichloroacetic acid (DCA) are 0.1 and 0.15 μg mL−1, respectively. The limits of quantification (LOQ) and the linear dynamic ranges (LDR) of MCA are found to be 1 and 1-400 μg mL−1, respectively, and for DCA are found to be 3 and 3-400 μg mL−1, respectively. The precision at the 5 ppm level for MCA and DCA are about 3% and 2%, (n = 5), respectively. The average recovery for MCA and DCA spiked to betaine samples are 98% and 97%, respectively. |
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Keywords: | HPLC Monochloroacetic acid Dichloroacetic acid 1-Naphthylamine Derivatization Betaine |
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