Use of anion-exchange high-performance liquid chromatography for the study of smooth muscle myosin light-chain kinase and its catalytic domain |
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| Authors: | L Dalla Libera M Fasolo P Cavallini E Ratti G Gaviraghi |
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| Institution: | National Research Council Unit for Muscle Biology and Physiopathology, Institute of General Pathology, Italy. |
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| Abstract: | Avian myosin light-chain kinase from smooth muscle of the gizzard and its catalytic domain, derived from the intact enzyme by trypsin digestion, was purified within 30-40 min by both analytical and preparative anion-exchange high-performance liquid chromatography. The proteins obtained were more than 95% pure and retained their biological activity. The high-performance anion-exchange chromatography protocols represent a significant decrease in purification time when compared with conventional ion-exchange chromatography. |
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