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Broadly tunable UV-blue picosecond pulsed laser and its application for biological imaging |
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| Authors: | Yasuhiro Maeda Masaki Yumoto Norihito Saito Takayo Ogawa Kazuo Kurokawa Akihiko Nakano Masafumi Yamashita Satoshi Wada |
| Institution: | 1. Department of Physics, Graduate School of Science, Tokyo University of Science, Shinjuku, Tokyo, 162-8601, Japan 2. Solid-State Laser for Astronomical Observation Research Team, RIKEN, Wako, Saitama, 351-0198, Japan 3. Molecular Membrane Biology Laboratory, RIKEN, Wako, Saitama, 351-0198, Japan 4. Department of Physics, Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba, 278-8510, Japan |
| Abstract: | We have achieved a rapid and random wavelength tuned picosecond pulsed laser and a widely tunable UV-blue picosecond pulsed laser by using the intracavity second harmonic generation of the laser. The tuning range was from 384 to 434 nm with picosecond pulse oscillation. In addition, we demonstrated biological imaging using a fluorescent protein excited by the widely tunable UV-blue picosecond pulsed laser. We found that the laser is suitable for biological imaging using the fluorescent protein as an excitation light source without damages. |
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