Characterization of single-stranded DNA separation by capillary gel electrophoresis |
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| Authors: | Moon Byoung GeounKyu Seong Choi Yong-Ill LeeYongseong Kim |
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| Institution: | a Division of Chemistry and Chemical Engineering, Kyungnam University, Masan 631-701, South Korea
b Department of Chemistry, College of Natural Sciences, Changwon National University Changwon, Kyungnam 641-773, South Korea |
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| Abstract: | We have investigated the effect of polymer gel reconditioning, the shape of the capillary, the applied electric field, and the capillary length for single-stranded DNA. The polyethylene oxide gel had deformed under the high electric field causing the degradation of the separation power. By the reintroduction of the fresh polyethylene oxide gel for the next run, one-base resolution was recovered. It turned out that the tip of the capillary at the injection side needed to be clean and symmetric for much improved resolution. Changing DNA motion by the pulsed electric field resulted in the separation of DNA far more than 500 bases. |
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| Keywords: | Single-stranded DNA Capillary gel electrophoresis PEO |
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